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Literature summary extracted from

  • Kurpiewska, K.; Font, J.; Ribo, M.; Vilanova, M.; Lewi?ski, K.
    X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: further insight into protein stability (2009), Proteins, 77, 658-669.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Escherichia coli strain BL21 Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.18 hanging drop vapor diffusion method, ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, crystal structure of bovine pancreatic ribonuclease A (wild-type), resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V47A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V54A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V57A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant I81A, resolution 2.0 A, crystal structure of bovine pancreatic ribonuclease A variant I106A, resolution 1.40 A, crystal structure of bovine pancreatic ribonuclease A variant V108A, resolution 1.60 A, space group P3221 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 I106A site-directed mutagenesis Bos taurus
4.6.1.18 I81A site-directed mutagenesis Bos taurus
4.6.1.18 additional information the structure of bovine pancreatic ribonuclease A variants V47A, V54A, V57A, I81A, I106A, and V108A is solved at 1.4-2.0 A resolution and compared with the structure of wild-type protein. The introduced mutations have only minor influence on the global structure of ribonuclease A. The structural changes have individual character that depends on the localization of mutated residue, however, they seem to expand from mutation site to the rest of the structure. Analysis of the difference distance matrices reveal that the ribonuclease A molecule is organized into five relatively rigid subdomains with individual response to mutation Bos taurus
4.6.1.18 V108A site-directed mutagenesis Bos taurus
4.6.1.18 V47A site-directed mutagenesis Bos taurus
4.6.1.18 V54A site-directed mutagenesis Bos taurus
4.6.1.18 V57A site-directed mutagenesis Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 pancreatic ribonuclease A
-
 Bos taurus